Chromatography Normal Phase and Reversed Phase
HPLC stands for High-Performance Liquid Chromatography formerly referred to as High-Pressure Liquid Chromatography. Normalreversed-phase chromatography separates molecules by their polarity. Chemistry Net Liquid Chromatography Hplc Separation Modes Ste Polymer Science Organic Chemistry Books Chemistry Help HIC acquires an advantage of the hydrophobic. . Cyano Phases For Separation And Extraction. Octadecyl C18 is the most common stationary phase but octyl C8 and butyl C4 are also used in some applications. Normal Phase Liqiuid Chromatography The stationary phase is polar and the mobile phase relatively non-polar. Ion pair chromatography IPC is an effective reversed-phase liquid chromatographic RPLC technique for separation of organic ions and partly ionized organic analytes. For reversed phase alkyl hydrocarbons are the preferred stationary phase. The technique utilizes the same types of stationary phases and mobile phases